產(chǎn)品編號 yb-1632R
英文名稱 Anti-APPL1抗體
中文名稱 銜接因子蛋白含pH域蛋白1抗體
別    名 Adapter protein containing PH domain; Adaptor Protein Containing pH Domain; Adaptor protein containing pH domain PTB domain and leucine zipper motif 1; Adaptor protein containing pH domain PTB domain and leucine zipper motif; AKT2 INTERACTOR; APPL 1; APPL1; DCC interacting protein 13 alpha; DCC-interacting protein 13-alpha; DIP 13 alpha; DIP13 alpha; Dip13-alpha; DIP13alpha; DP13A_HUMAN; KIAA1428; PTB domain and leucine zipper motif 1; PTB Domain and Leucine Zipper Motif; Signaling adaptor protein DIP13alpha.
 Anti-APPL1抗體   
說 明 書 0.1ml  0.2ml  
研究領域 心血管  細胞生物  發(fā)育生物學  神經(jīng)生物學  信號轉導  
抗體來源 Rabbit
克隆類型 Polyclonal
交叉反應 Human, Mouse, Rat, Dog, Pig, Cow, Horse, Rabbit, 
產(chǎn)品應用 WB=1:100-500 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500 （石蠟切片需做抗原修復） 
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
分 子 量 80kDa
細胞定位 細胞核 細胞漿 細胞膜 
性    狀 Lyophilized or Liquid
濃    度 1mg/1ml
免 疫 原 KLH conjugated synthetic peptide derived from human APPL1
亞    型 IgG
純化方法 affinity purified by Protein A
儲 存 液 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存條件 Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
 
PubMed PubMed
產(chǎn)品介紹 background:
The protein encoded by this gene has been shown to be involved in the regulation of cell proliferation, and in the crosstalk between the adiponectin signalling and insulin signalling pathways. The encoded protein binds many other proteins, including RAB5A, DCC, AKT2, PIK3CA, adiponectin receptors, and proteins of the NuRD/MeCP1 complex. This protein is found associated with endosomal membranes, but can be released by EGF and translocated to the nucleus. [provided by RefSeq, Jul 2008]

Function:
Required for the regulation of cell proliferation in response to extracellular signals from an early endosomal compartment. Links Rab5 to nuclear signal transduction.

Subunit:
Binds RAB5A/Rab5 through an N-terminal domain. This interaction is essential for its recruitment to endosomal membranes as well as its role in cell proliferation. Binds DCC and the catalytic domain of the inactive form of AKT2 through its PID domain. Binds PIK3CA and subunits of the NuRD/MeCP1 complex. Interacts with OCRL and INPP5B.

Subcellular Location:
Early endosome membrane; Peripheral membrane protein. Nucleus. Note=Early endosomal membrane-bound and nuclear. Translocated into the nucleus upon release from endosomal membranes following internalization of EGF.

Tissue Specificity:
High levels in heart, ovary, pancreas and skeletal muscle.

Post-translational modifications:
Phosphorylated upon DNA damage, probably by ATM or ATR.
Phosophorylation at Ser-410 by PKA severely impairs binding to OCRL.

Similarity:
Contains 1 PH domain.
Contains 1 PID domain.

 
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. 

銜接因子蛋白含pH域磷酸*結合域和*拉鏈基元1抗體.
可調(diào)節(jié)脂聯(lián)素和胰島素信號，是細胞膜與細胞核之間重要的信息傳遞蛋白,同時也是細胞增殖所必需的蛋白，它有調(diào)節(jié)脂聯(lián)素在脂肪酸氧化和葡萄糖吸收中的作用，APPL1通過激酶通道來調(diào)節(jié)脂聯(lián)素的胰島素敏感效應。