產品編號 YB-2412R
英文名稱 Anti-APNG/MPG抗體
中文名稱 N-甲基嘌呤DNA糖基化酶
別    名 MPG; N-methylpurine-DNA glycosylase isoform a; DNA 3 methyladenine glycosylase; 3 alkyladenine DNA glycosylase; AAG; ADPG; Alkyladenine DNA glycosylase; anpg ; APNG; MDG; N methylpurine DNA glycosirase; CRA36.1; MDG; Mid1; PIG11; PIG16; 3MG_HUMAN.
Anti-APNG/MPG抗體    
說 明 書 0.1ml  0.2ml  
研究領域 細胞生物  染色質和核信號  表觀遺傳學  
抗體來源 Rabbit
克隆類型 Polyclonal
交叉反應 Human, Mouse, Rat, Pig, Cow, 
產品應用 WB=1:100-500 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500 （石蠟切片需做抗原修復） 
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
分 子 量 33kDa
細胞定位 細胞核 
性    狀 Lyophilized or Liquid
濃    度 1mg/1ml
免 疫 原 KLH conjugated synthetic peptide derived from human APNG/MPG
亞    型 IgG
純化方法 affinity purified by Protein A
儲 存 液 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存條件 Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.

PubMed PubMed
產品介紹 background:
MPG (N-methylpurine DNA glycosylase, or 3-methyladenine (3MeA) DNA glycosylase) repairs the 3MeA lethal lesion blocking DNA replication in Escherichia coli. Analysis of human cDNA libraries found a cDNA that would hybridize to human genomic DNA but not to E. coli or yeast DNA. This MPG gene has been mapped to human chromosome 16 by analysis of a panel of DNAs from mouse/human and hamster/human hybrid cell lines.

Function:
Hydrolysis of the deoxyribose N-glycosidic bond to excise 3-methyladenine, and 7-methylguanine from the damaged DNA polymer formed by alkylation lesions.

Subunit:
Belongs to the DNA glycosylase MPG family.

Subcellular Location:
Nucleus.

Similarity:
Belongs to the DNA glycosylase MPG family.


Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.