產(chǎn)品編號(hào) yb-2720R
英文名稱 Anti-phospho-Akt(Thr308)抗體
中文名稱 磷酸化蛋白激酶B抗體
別    名 AKT (phospho T308); p-AKT (phospho T308); Akt(Phospho-Thr308); Akt(Phospho T308); C AKT; MGC9965; Oncogene AKT1; PKB; PRKBA; Protein Kinase B Alpha; RAC Alpha; RAC; RAC PK Alpha; RAC Serine/Threonine Protein Kinase; vAKT Murine Thymoma Viral Oncogene Homolog 1; AKT1_HUMAN.
 Anti-phospho-Akt(Thr308)抗體  
說(shuō) 明 書 0.1ml  
產(chǎn)品類型 磷酸化抗體 
研究領(lǐng)域 細(xì)胞生物  神經(jīng)生物學(xué)  信號(hào)轉(zhuǎn)導(dǎo)  細(xì)胞凋亡  細(xì)胞周期蛋白  激酶和磷酸酶  
抗體來(lái)源 Rabbit
克隆類型 Polyclonal
交叉反應(yīng) Human, Mouse, Rat, Chicken, Dog, Pig, Cow, Rabbit, Sheep, 
產(chǎn)品應(yīng)用 WB=1:100-500 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500 （石蠟切片需做抗原修復(fù)） 
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
分 子 量 56kDa
細(xì)胞定位 細(xì)胞核 細(xì)胞漿 細(xì)胞膜 
性    狀 Lyophilized or Liquid
濃    度 1mg/1ml
免 疫 原 KLH conjugated Synthesised phosphopeptide derived from human Akt around the phosphorylation site of Thr308
亞    型 IgG
純化方法 affinity purified by Protein A
儲(chǔ) 存 液 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存條件 Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
 Anti-phospho-Akt(Thr308)抗體
PubMed PubMed
產(chǎn)品介紹 background:
The serine-threonine protein kinase encoded by the AKT1 gene is catalytically inactive in serum-starved primary and immortalized fibroblasts. AKT1 and the related AKT2 are activated by plaet-derived growth factor. The activation is rapid and specific, and it is abrogated by mutations in the pleckstrin homology domain of AKT1. It was shown that the activation occurs through phosphatidylinositol 3-kinase. In the developing nervous system AKT is a critical mediator of growth factor-induced neuronal survival. Survival factors can suppress apoptosis in a transcription-independent manner by activating the serine/threonine kinase AKT1, which then phosphorylates and inactivates components of the apoptotic machinery. Mutations in this gene have been associated with the Proteus syndrome. Multiple alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Jul 2011]

Subunit:
Interacts (via PH domain) with TCL1A; this enhances AKT3 phosphorylation and activation. Interacts with TRAF6.

Subcellular Location:
Cytoplasm. Nucleus. Cell membrane. Note=Nucleus after activation by integrin-linked protein kinase 1 (ILK1). Nuclear translocation is enhanced by interaction with TCL1A. Phosphorylation on Tyr-176 by TNK2 results in its localization to the cell membrane where it is targeted for further phosphorylations on Thr-308 and Ser-473 leading to its activation and the activated form translocates to the nucleus.

Tissue Specificity:
Widely expressed. Low levels found in liver with slightly higher levels present in thymus and testis.

Similarity:
Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. RAC subfamily. 
Contains 1 AGC-kinase C-terminal domain. 
Contains 1 PH domain. 
Contains 1 protein kinase domain.

 Anti-phospho-Akt(Thr308)抗體
激酶和磷酸酶（Kinases and Phosphatases） Akt/PKB(Protein kinase B;Protein kinase beta, gamma;PKB gamma;STK-2 )是*/*蛋白激酶家族成員，可使蛋白磷酸化，也可自身磷酸化,在調(diào)節(jié)細(xì)胞存活中起重要作用。 Akt1/PKBa是一種*/*蛋白激酶，其激酶活力在包括細(xì)胞凋亡、糖原合成和細(xì)胞生長(zhǎng)等的各種細(xì)胞功能中起著關(guān)鍵作用。Akt1/PKBa 被各種生長(zhǎng)因子和存活因子所活化。其308位*殘基、473位*殘基和474位*殘基的磷酸化對(duì)于該酶的充分活化是*的。Akt1/PKBa通過(guò)對(duì)包括胱冬酶-9在內(nèi)的幾種靶標(biāo)的磷酸化和失活而抑制細(xì)胞凋亡。 Akt1/PKBa 通過(guò)對(duì)糖原合酶激酶-3a和3b的磷酸化和失活來(lái)調(diào)節(jié)糖原合成。已鑒定了3種亞型的哺乳動(dòng)物Akt1/PKBa。在很多種癌癥中AKT都有非正常表達(dá)或過(guò)表達(dá)。 Murine thymoma viral(v-akt) oncogene homolog-2(AKT-2;PRKBB)—蛋白激酶AKT-2，被認(rèn)為也是原癌基因之一。受PDGF，EGF，F(xiàn)GF激活，經(jīng)過(guò)phosphatidylinositol 3-kinase作用而活化。通過(guò)對(duì)凋亡調(diào)控蛋白的磷酸化而滅活其活性，抑制細(xì)胞的凋亡。