產(chǎn)品編號 bs-3777R
英文名稱 Anti-ASF1A抗體
中文名稱 細胞衰老相關(guān)蛋白ASF1A抗體
別    名 Anti silencing function 1A; ASF1 anti silencing function 1 homolog A; CCG1 interacting factor A; CG9383; CGI 98; CGI98 protein; CIA; dASF1; hAsf1; hAsf1a; hCIA; Histone chaperone ASF1A; HSPC146; ASF1A_HUMAN.
Anti-ASF1A抗體 
說 明 書 0.1ml  0.2ml  
研究領(lǐng)域 腫瘤  細胞生物  免疫學  染色質(zhì)和核信號  轉(zhuǎn)錄調(diào)節(jié)因子  
抗體來源 Rabbit
克隆類型 Polyclonal
交叉反應(yīng) Human, Mouse, Rat, Pig, Cow, 
產(chǎn)品應(yīng)用 WB=1:100-500 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500 （石蠟切片需做抗原修復(fù)） 
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
分 子 量 23kDa
細胞定位 細胞核 
性    狀 Lyophilized or Liquid
濃    度 1mg/1ml
免 疫 原 KLH conjugated synthetic peptide derived from human ASF1A
亞    型 IgG
純化方法 affinity purified by Protein A
儲 存 液 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存條件 Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.

PubMed PubMed
產(chǎn)品介紹 background:
ASF1a belongs to the H3/H4 family of histone chaperone proteins. The protein is a key component of a histone donor complex that functions in nucleosome assembly. It interacts with histones H3 and H4, and functions together with a chromatin assembly factor during DNA replication, repair and silencing.

Function:
Histone chaperone that facilitates histone deposition and histone exchange and removal during nucleosome assembly and disassembly. Cooperates with chromatin assembly factor 1 (CAF-1) to promote replication-dependent chromatin assembly and with HIRA to promote replication-independent chromatin assembly. Required for the formation of senescence-associated heterochromatin foci (SAHF) and efficient senescence-associated cell cycle exit.

Subunit:
Interacts with histone H3 (including both histone H3.1 and H3.3) and histone H4. Interacts with the CHAF1A, CHAF1B and RBBP4 subunits of the CAF-1 complex. Interacts with CABIN1, HAT1, HIRA, NASP, TAF1, TLK1, TLK2 and UBN1. Interacts with CDAN1. Found in a cytosolic complex with CDAN1, ASF1B, IPO4 and histones H3.1 and H4.

Subcellular Location:
Nucleus.

Tissue Specificity:
Ubiquitously expressed.

Post-translational modifications:
Phosphorylated by TLK1 and TLK2. Highly phosphorylated in S-phase and at lower levels in M-phase. TLK2-mediated phosphorylation at Ser-192 prevents proteasome-dependent degradation.

Similarity:
Belongs to the ASF1 family.

Database links:
UniProtKB/Swiss-Prot: Q9Y294.1

Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. 
能夠促進細胞衰老并且是與衰老有關(guān)的細胞周期停滯所必須的。HIRA/ASF1a介導的SAHF的形成過程直接促進細胞脫離細胞周期循環(huán)進入衰老階段。研究人員還推測HIRA和ASF1a的改變可能使細胞無法脫離細胞周期并可能導致癌癥的發(fā)生。